RNA

Part:BBa_K5319669:Design

Designed by: YuFei Xie   Group: iGEM24_ZJU-China   (2024-09-30)


HYER-J2/3


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 361
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 361
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 361
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The target site of mutation is chosen referring to the idea from "Crystal structure of a group II intron in the pre-catalytic state" to stablize the RNA in the pre-catalytic state by mutation, which creates a spatial shift in three dimensional structure.


Source

HYER(hydrolytic endonucleolytic ribozymes), identified from group II-C introns in public bacterial genome database in the article "Hydrolytic endonucleolytic ribozyme (HYER) is programmable for sequence-specific DNA cleavage".

References